Certification Board for Sterile Processing and Distribution (CBSPD) Sterile Processing Technician Practice Exam

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Which microorganism is used to biologically monitor the Low Temperature Gas Plasma (LTGP) sterilizer?

  1. Bacillus subtilis

  2. Geobacillus stearothermophilus

  3. Clostridium sporogenes

  4. Escherichia coli

The correct answer is: Geobacillus stearothermophilus

The correct choice is Geobacillus stearothermophilus, which is used to biologically monitor Low Temperature Gas Plasma (LTGP) sterilizers. This microorganism is a highly resistant, heat-stable spore-forming bacillus that is specifically chosen for its ability to withstand the sterilization conditions provided by LTGP systems. The spore’s resistance characteristics make it an ideal biological indicator to assess the effectiveness of the sterilization process, ensuring that the LTGP system is functioning correctly and achieving the necessary lethality to confirm sterility. In the context of sterilization monitoring, using Geobacillus stearothermophilus allows technicians to validate that the sterilization cycle has effectively eliminated viable microorganisms, which is a crucial component of infection control in healthcare settings. The presence of these spores in the sterilization process serves as a vital safety measure, helping to maintain high standards of patient safety and equipment sterility.